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ALP, <t>BMP2,</t> RUNX2, OCN, and OPN mRNA expression at day 7 ( A ) and at day 14 ( B ). C Representative western blots of ALP, BMP2, COL-1, and RUNX2 expression at day 14 and quantification ( D ). E The images of the frozen sections obtained from microspheroids cultured at 14 days under osteogenic medium with or without CBD stained with immunofluorescence and F quantification. Data were presented as the mean ± SD ( n = 3). Significant difference compared different groups, * P < 0.05, ** P < 0.01, and *** P < 0.001; compared between DPSC and CBD+DPSC groups in blue; compared between DPSC and spheroids groups in red; compared between spheroids and CBD+spheroids groups in green; compared between CBD+DPSC and CBD+spheroids groups in purple.
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ALP, <t>BMP2,</t> RUNX2, OCN, and OPN mRNA expression at day 7 ( A ) and at day 14 ( B ). C Representative western blots of ALP, BMP2, COL-1, and RUNX2 expression at day 14 and quantification ( D ). E The images of the frozen sections obtained from microspheroids cultured at 14 days under osteogenic medium with or without CBD stained with immunofluorescence and F quantification. Data were presented as the mean ± SD ( n = 3). Significant difference compared different groups, * P < 0.05, ** P < 0.01, and *** P < 0.001; compared between DPSC and CBD+DPSC groups in blue; compared between DPSC and spheroids groups in red; compared between spheroids and CBD+spheroids groups in green; compared between CBD+DPSC and CBD+spheroids groups in purple.
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ALP, BMP2, RUNX2, OCN, and OPN mRNA expression at day 7 ( A ) and at day 14 ( B ). C Representative western blots of ALP, BMP2, COL-1, and RUNX2 expression at day 14 and quantification ( D ). E The images of the frozen sections obtained from microspheroids cultured at 14 days under osteogenic medium with or without CBD stained with immunofluorescence and F quantification. Data were presented as the mean ± SD ( n = 3). Significant difference compared different groups, * P < 0.05, ** P < 0.01, and *** P < 0.001; compared between DPSC and CBD+DPSC groups in blue; compared between DPSC and spheroids groups in red; compared between spheroids and CBD+spheroids groups in green; compared between CBD+DPSC and CBD+spheroids groups in purple.

Journal: Communications Biology

Article Title: Dental pulp stem cells-derived cannabidiol-treated organoid-like microspheroids show robust osteogenic potential via upregulation of WNT6

doi: 10.1038/s42003-024-06655-y

Figure Lengend Snippet: ALP, BMP2, RUNX2, OCN, and OPN mRNA expression at day 7 ( A ) and at day 14 ( B ). C Representative western blots of ALP, BMP2, COL-1, and RUNX2 expression at day 14 and quantification ( D ). E The images of the frozen sections obtained from microspheroids cultured at 14 days under osteogenic medium with or without CBD stained with immunofluorescence and F quantification. Data were presented as the mean ± SD ( n = 3). Significant difference compared different groups, * P < 0.05, ** P < 0.01, and *** P < 0.001; compared between DPSC and CBD+DPSC groups in blue; compared between DPSC and spheroids groups in red; compared between spheroids and CBD+spheroids groups in green; compared between CBD+DPSC and CBD+spheroids groups in purple.

Article Snippet: The membrane was then incubated at 4 °C overnight with primary antibodies, which included rabbit anti-ALP (Affinity, China), rabbit anti-BMP2 (Abcam, England), rabbit anti-OCN (Abcam, England), rabbit anti-human Collagen I (COL-I, Abcam, England), rabbit anti-human RUNX2 (Abcam, England), rabbit anti-human WNT6 (Bioss, China), and rabbit anti-human β-catenin (Bioss, China).

Techniques: Expressing, Western Blot, Cell Culture, Staining, Immunofluorescence

Primers used in RT-qPCR to examine gene expression

Journal: Communications Biology

Article Title: Dental pulp stem cells-derived cannabidiol-treated organoid-like microspheroids show robust osteogenic potential via upregulation of WNT6

doi: 10.1038/s42003-024-06655-y

Figure Lengend Snippet: Primers used in RT-qPCR to examine gene expression

Article Snippet: The membrane was then incubated at 4 °C overnight with primary antibodies, which included rabbit anti-ALP (Affinity, China), rabbit anti-BMP2 (Abcam, England), rabbit anti-OCN (Abcam, England), rabbit anti-human Collagen I (COL-I, Abcam, England), rabbit anti-human RUNX2 (Abcam, England), rabbit anti-human WNT6 (Bioss, China), and rabbit anti-human β-catenin (Bioss, China).

Techniques: Sequencing